Coding

Part:BBa_K1112002

Designed by: Alba Iglesias Vilches   Group: iGEM13_Valencia_Biocampus   (2013-09-10)

Cluster PHA

Cluster for PHA production in P. putida has a complete sequence of 4978 bp. The operon consists of three genes: from 409-2088 bp codifies for gene phaC1 which product is the PHA synthase 1. From 2151-3008 bp phaZ gene is codified, and its product is the PHA depolymerase. Finally, from 3027-4709 bp gene phaC2 codifies for the PHA synthase 2.

Usage and Biology

PHA accumulates under unbalanced growth conditions as a means of intracellular storage, storing excess carbon and energy. These PHA polymers are synthesized by enzyme PHA synthase which is bound to the surface of the PHA granules and uses coenzyme A thioesters of hydroxyalkanoic acids as substrates.

Kinetics of the PHA production and biomass generation were realized in relation to the time:

Figure 1. Kinetics of production of PHA and biomass generation: Left axis, biomass concentration (cell dry weight) Right axis, PHA concentration (measured gravimetrically)


Figure 2. PHA extraction and detection with red Nile.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
    Illegal NotI site found at 3106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1819
    Illegal XhoI site found at 2271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1620
    Illegal NgoMIV site found at 1747
    Illegal NgoMIV site found at 1996
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 3915
    Illegal NgoMIV site found at 4368
    Illegal AgeI site found at 374
    Illegal AgeI site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


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